diauxic growth

Trait evidence (5)

• DOI:10.1371/journal.pone.0061686

  However, a significant fraction of cells maintains a high rate of elongation and displays no detectable growth lag during the shift.

  Boulineau et al. 2013, PMID:23637881, PMC3640066; scientific abstract. Introduction defines diauxic growth by sequential carbon-source use, supporting the definition's nutrient-switching differentia. Relevant Results, Discussion, Methods, actual Figure 2 and Text S1 prose were inspected. AB460 is an engineered MG1655 derivative; single-cell medium exchange is not the same assay as mixed-substrate batch growth. Population lag does not imply arrest of every cell. Contaminant-supported growth and model assumptions remain relevant. Text S1 DOC extraction loses embedded equations; no equation was visually verified or used as a curated mechanistic claim.

• DOI:10.1128/jb.164.2.866-871.1985

  Azotobacter vinelandii exhibited diauxie when grown in a medium containing both acetate and glucose as carbon sources.

  George et al. 1985, PMID:3863813, PMC214331; directly retrieved source-qualified MED abstract only. Acetate preference in this experiment contradicts a universal glucose-first requirement and supports the lexical name diauxie. Full paper and figures were not inspected; a full-text XML endpoint returned HTTP 500. This access failure is not evidence that full text does not exist.

• DOI:10.1128/jb.01571-06

  Following recovery of growth on the alternative carbon or nitrogen source, RpoS protein concentrations declined rapidly to a basal level.

  Sandercock and Page 2008, PMID:18055600, PMC2223558; scientific abstract. Relevant Methods/Results and actual Figure 3 support UW batch-culture acetate/glucose and ammonium/N2 transitions. This supports nutrient-source scope rather than sugars or carbon alone. Ammonium augmentation addresses an oxygen-limitation alternative for the stress response. RpoS dynamics are not a universal causal mechanism of diauxie; later PHB consumption was explicitly unmeasured. Publisher text and Figure 3 were accessible despite requests HTML 403 and Europe PMC XML 500 responses.

• DOI:10.1371/journal.pbio.1002041

  many natural isolates of Saccharomyces cerevisiae display short or nonexistent diauxic lags when grown in mixtures of glucose (preferred) and galactose.

  Wang et al. 2015, PMID:25626068, PMC4308108; scientific abstract, not the XML's preceding TOC teaser or separate Author Summary. Relevant Results, growth-curve Methods, actual Figure 1 and S1 EPS were inspected. Operational zero lag means the smoothed growth-rate curve has no local minimum, not simultaneous equal use of both substrates. Natural-origin growth panels and engineered reporter derivatives require separate provenance. Figure 1 caption says eleven strains but the image has twelve labels; no strain count is inferred here. The observations oppose mandatory positive lag.

• DOI:10.1038/msb.2013.11

  Once glucose is depleted, the cells consume the earlier produced ethanol

  Zampar et al. 2013, PMID:23549479, PMC3693829; Introduction, second paragraph, not an abstract quote. Relevant Results, culture and measurement Methods and actual Figure 1 were inspected. FY4 was initially supplied glucose and later consumed produced ethanol; the definition therefore does not require both nutrients to be supplied initially. Figure 1 distinguishes concentration observations from fitted rates. Intracellular flux ranges are model-constrained, not direct flux measurements; raw supplemental data and the full mechanistic analysis were not reanalyzed.