TraitMech

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pyrazinamidase activity

traitmech:000163 · CLASS · PROPOSED

A physiological enzyme-activity phenotype in which a cell produces active nicotinamidase/pyrazinamidase enzymes that hydrolyze nicotinamide and can convert pyrazinamide to pyrazinoic acid.

Trait evidence (3)

  • https://iubmb.qmul.ac.uk/enzyme/EC3/5/1/19.html
    <b>Reaction:</b> nicotinamide + H<small><sub>2</sub></small>O = nicotinate + NH<small><sub>3</sub></small>

    The NC-IUBMB EC 3.5.1.19 entry grounds the nicotinamide-deamidating side of the PncA activity with the accepted nicotinamidase reaction.

  • DOI:10.1111/j.1742-4658.2007.06241.x
    The nicotinamidase/pyrazinamidase (PncA) of Mycobacterium tuberculosis is involved in the activation of the important front-line antituberculosis drug pyrazinamide by converting it into the active form, pyrazinoic acid.

    Zhang et al. characterized the Mycobacterium tuberculosis PncA enzyme, linking nicotinamidase/pyrazinamidase activity to cellular conversion of pyrazinamide into pyrazinoic acid.

  • DOI:10.1371/journal.pone.0015785
    Pyrazinamidase (PncA) activates the first-line antituberculous drug pyrazinamide into pyrazinoic acid.

    Petrella et al. independently describe PncA as the pyrazinamidase that activates pyrazinamide, supporting the pyrazinamidase label for the organism-level activity.

Provenance

Identifier source
TraitMech local identifier
Definition source
DOI:10.1111/j.1742-4658.2007.06241.x

Parent traits (1)

Synonyms (4)

  • nicotinamidase activity RELATED_SYNONYM · https://iubmb.qmul.ac.uk/enzyme/EC3/5/1/19.html
  • nicotinamidase RELATED_SYNONYM · https://iubmb.qmul.ac.uk/enzyme/EC3/5/1/19.html
  • nicotinamide amidohydrolase RELATED_SYNONYM · https://iubmb.qmul.ac.uk/enzyme/EC3/5/1/19.html
  • nicotinamidase/pyrazinamidase RELATED_SYNONYM · DOI:10.1111/j.1742-4658.2007.06241.x

kg-microbe context

No kg-microbe node embedding matched this record in the 2026-04-25 deepwalk.

Canonical examples (1)

Organisms cited as exemplars of this trait. Taxon ids are NCBITaxon and link out to the NCBI record.

Discussions and Knowledge Gaps (1)

Open questions attached to this trait. Seeded by just knowledge-gap-scan and curated; see the corpus-wide index.

Resolve an exact external ontology class for pyrazinamidase activity before adding a TraitRecord xref.

CURATION TODO OPEN pyrazinamidase-activity-xref-gap · raised by codex · 2026-09-12

Not yet attached to a section of this record — a curator sets attaches_to (e.g. causal_graphs#some_edge) so the gap shows beside the mechanism it concerns.

GO:0008936 nicotinamidase activity denotes the catalytic molecular function rather than the organism-level pyrazinamidase/nicotinamidase phenotype, so it is appropriate as a causal-node grounding for the source label and not as an equivalent TraitRecord xref.

Curation history

  1. · MINTED_TRAITMECH_ID · codex

    Minted pyrazinamidase activity as a URL/DOI-backed TraitRecord after a repository-wide duplicate review covering ignored and hidden files; METPO has no exact pyrazinamidase activity class yet.