TraitMech

TraitMech › PHYSIOLOGY

trypsin activity

traitmech:000158 · CLASS · PROPOSED

A physiological enzyme-activity phenotype in which a cell exhibits trypsin-like serine endopeptidase activity, preferentially cleaving peptide bonds on the carboxyl side of arginine or lysine residues.

Trait evidence (3)

  • https://iubmb.qmul.ac.uk/enzyme/EC3/4/21/4.html
    <b>Reaction:</b> Preferential cleavage: Arg<img src="../../../EZgif/FISS.GIF" align=top>, Lys<img src="../../../EZgif/FISS.GIF" align=top>

    The NC-IUBMB EC 3.4.21.4 entry accepts the name trypsin and its reaction line places the scissile-bond FISS.GIF image immediately after Arg and Lys.

  • https://iubmb.qmul.ac.uk/enzyme/EC3/4/21/4.html
    Isolated as multiple cationic and anionic trypsins [5] from the pancreas of many vertebrates and from lower species including crayfish, insects (cocoonase) and microorganisms

    The NC-IUBMB EC 3.4.21.4 comments note that trypsins have also been isolated from microorganisms.

  • DOI:10.1371/journal.pone.0322500
    Enzyme activities of acid- and alkaline-phosphatases, esterase, esterase lipase, leucine arylamidase, trypsin, naphthol-AS-BI phosphohydrolase, and alpha-glucosidase are present when assayed with API ZYM system.

    Park et al. treated trypsin as a positive API ZYM enzyme-activity phenotype in the Flexibacterium corallicola type species description.

Provenance

Identifier source
TraitMech local identifier
Definition source
https://iubmb.qmul.ac.uk/enzyme/EC3/4/21/4.html

Parent traits (1)

Synonyms (1)

  • trypsin RELATED_SYNONYM · https://iubmb.qmul.ac.uk/enzyme/EC3/4/21/4.html

kg-microbe context

No kg-microbe node embedding matched this record in the 2026-04-25 deepwalk.

Canonical examples (1)

Organisms cited as exemplars of this trait. Taxon ids are NCBITaxon and link out to the NCBI record.

Discussions and Knowledge Gaps (1)

Open questions attached to this trait. Seeded by just knowledge-gap-scan and curated; see the corpus-wide index.

Resolve an exact external ontology class for trypsin activity before adding a TraitRecord xref.

CURATION TODO OPEN trypsin-activity-xref-gap · raised by codex · 2026-09-12

Not yet attached to a section of this record — a curator sets attaches_to (e.g. causal_graphs#some_edge) so the gap shows beside the mechanism it concerns.

GO:0004295 trypsin activity is obsolete, and its replacement GO:0004252 denotes broader serine-type endopeptidase molecular function rather than the organism-level trypsin-like serine endopeptidase phenotype. GO:0004252 is appropriate as a causal-node grounding for generic serine endopeptidase activity, not as an equivalent TraitRecord xref.

Curation history

  1. · MINTED_TRAITMECH_ID · codex

    Minted trypsin activity as a URL/DOI-backed TraitRecord after a repository-wide duplicate review covering ignored and hidden files; METPO has no exact trypsin activity class yet.

  2. · CORRECTED_EVIDENCE_SNIPPET · codex

    Corrected the IUBMB reaction-line snippet for trypsin activity so it preserves the scissile-bond image tags from the source HTML (#819).

  3. · TIGHTENED_DEFINITION_WORDING · codex

    Tightened the trypsin activity definition to describe a trypsin-like organismal phenotype instead of over-specifying production of the named vertebrate enzyme (#820).