unequal protein aggregate inheritance at cell division

Trait evidence (4)

• DOI:10.1073/pnas.0708931105

  their position in the mother cell determines which offspring inherits the inclusion body and where it will be located within it

  Lindner et al. 2008, PMID:18287048, PMC2268587; Results, Inclusion Bodies Are Formed in Discrete Cellular Positions and Accumulate in Old Poles Under Nonstressing Conditions. Primary PMC Results and Methods directly read. The quoted position is that of an IbpA-YFP focus. MGAY is an engineered MG1655 reporter strain, grown on LB-agarose at 37 C without imposed stress. Figure 2 is the inspected localization measurement; Figure 3 is an inspected lineage schematic, not another experiment. Pole identity changes across divisions. These observations describe reporter-focus inheritance and eventual older-pole accumulation. Govers et al. 2018 (DOI:10.1371/journal.pbio.2003853) subsequently demonstrated label-induced clustering with IbpA-YFP. The older readout is not independent proof of endogenous unstressed aggregation; naming the engineered strain alone does not resolve this assay bias. Neither active transport, universal aggregate toxicity nor a natural canonical exemplar is established. Supplements were not inspected. The full-text span retains the native abstract resolver verdict NOT_IN_ABSTRACT.

• DOI:10.1371/journal.pbio.1001886

  We experimentally confirmed that fusion increases segregation asymmetry, for a range of stresses, and identified Hsp16 as a fusion factor.

  Coelho et al. 2014, PMID:24936793, PMC4061010; scientific abstract, not Author Summary. Primary XML and actual Figures 3, 4 and S4 were inspected. Hsp104-GFP fission-yeast imaging supports stress-associated unequal inheritance without consistent old/new-pole bias. Figure 3C measures an absolute sibling difference in aggregate-associated fluorescence, not concentration; S4I measures count asymmetry. Figure 4B is fusion and 4C is nucleation: the caption reverses them. A Results pointer to 4C for stress-dependent asymmetry disagrees with the plotted endpoints. Figure 3C describes continuous 40 C, whereas Methods describes 40 C for one hour followed by 37 C; this remains unresolved. Text S1 experimental methods and strain table were text-extracted, not visually rendered. No universal transport, death threshold or rejuvenation mechanism is inferred. The scientific-abstract span matches the native resolver text (VERIFIED).

• DOI:10.1111/mmi.14228

  Noticeably, stalked cells always inherited more aggregates (75%) than swarmer cells (25%).

  Schramm et al. 2019, PMID:30779464, PMC6850343; Results, The aggregate load does not sort to swarmer or stalked daughter cells in C. crescentus. Primary XML Results, Discussion and relevant Methods were read; actual Figure 6 was inspected. DnaK-mVenus, delta-clpB cells recovered at 30 C after 10 minutes at 40 C. Figure 6G reports aggregate allocation across daughter cell types over successive divisions, not the fraction of all sibling pairs with unequal counts. Figure 6F measures projected cell area, not volume. Unequal shared inheritance is compatible with the broad phenotype; it contradicts neither sharing nor a stable allocation ratio. The paper limits universal exclusive old-pole retention, not every form of quantitative inequality. Supplements and independent strain provenance were not inspected; no natural canonical example is asserted. The full-text span retains the native abstract resolver verdict NOT_IN_ABSTRACT.

• DOI:10.1371/journal.pbio.2003853

  the polarly deposited PA was subjected to limited disaggregation and therefore became asymmetrically inherited for a large number of generations

  Govers et al. 2018, PMID:30153247, PMC6112618; scientific abstract, directly matched in primary XML and source-qualified MED. Results on reporter validation and post-stress inheritance, relevant Methods and actual Figures 1 and 3 were inspected. Figure 1 shows reporter-dependent clustering; Figure 3 separately follows engineered MG1655 IbpA-msfGFP lineages after 47 C for 15 minutes, with microscopy at 37 C. Focus inheritance is distinct from the fluorescence concentration and growth readouts. This does not validate unstressed IbpA-YFP foci as endogenous aggregates or imply universal harm, benefit, aging or rejuvenation. Supplementary controls were read only where described in Results; their images, raw data and independent strain provenance were not assessed.