NaCl optimum high

Edge evidence

• hypersaline NaCl selects for salt-in strategy METPO:2007401

  Hypersaline conditions select for intracellular ion-accumulation osmoadaptation.

  • DOI:10.1186/1746-1448-4-2 KCl accumulating Halobacterium salinarum Supports the salt-in strategy in extreme-halophile lineages.
• salt-in strategy produces NaCl optimum high METPO:2000202

  Salt-in ion accumulation yields a >8% NaCl-optimum setpoint.

  • DOI:10.1093/femsre/fuy009 extreme halophile Supports the >8% optimum as the extreme-halophile outcome.
• NaCl optimum high is a NaCl optimum rdfs:subClassOf

  NaCl optimum high is a quantitative bin of the NaCl-optimum phenotype.

  • DOI:10.1093/femsre/fuy009 optimal NaCl Supports >8% optimum as a value within the NaCl-optimum distribution.
• salt-in strategy increases intracellular KCl concentration RO:0002213

  Salt-in osmoadaptation accumulates high intracellular KCl.

  • DOI:10.1002/pro.5003 Halophiles accumulate intracellular concentrations of potassium chloride even above 3 M.
• salt-in strategy requires potassium uptake system

  Salt-in strategy depends on K+ uptake systems to accumulate cytoplasmic potassium.

  • DOI:10.1002/pro.5003 Upregulating potassium-selective ion channels supports salt-in K+ accumulation.
• Na+/H+ antiporter supports salt-in strategy

  Na+/H+ antiporter activity supports salt-in osmoadaptation via Na+ exclusion.

  • DOI:10.3390/microorganisms12081738 Na+ extrusion via Na+/H+ antiporters driven by a proton electrochemical gradient.
• intracellular KCl concentration selects for acidic proteome METPO:2007401

  High intracellular KCl selects for an acidic proteome.

  • DOI:10.1038/s41559-024-02505-6 The salt-in strategy is concomitant with an excess of acidic amino acids.
• acidic proteome enables protein function at high salinity RO:0002327

  Acidic proteome enables protein function at high salinity.

  • DOI:10.1038/s41559-024-02505-6 Proteome acidification preserves protein structure/function under high intracellular salt.
• increased acidic amino-acid content increases protein solubility in high salt RO:0002213

  Increased acidic amino-acid content increases protein solubility in high salt.

  • DOI:10.1002/pro.5003 Increase the number of short, polar and acidic amino acids to improve salt-induced stabilization and solubility.