NaCl range high

Edge evidence

• salt-in physiology produces NaCl range high METPO:2000202

  Salt-in physiology yields a growth range extending above 8% NaCl.

  • DOI:10.1186/1746-1448-4-2 KCl accumulating Halobacterium salinarum Supports salt-in physiology as the basis of extreme-halophile growth ranges.
• NaCl range high is a NaCl range rdfs:subClassOf

  NaCl range high is a quantitative bin of the NaCl-range phenotype.

  • DOI:10.1093/femsre/fuy009 salinity range Supports the >8% range as a value within the NaCl-range distribution.
• high external NaCl / hypersaline brine induces intracellular K+

  High external NaCl induces molar intracellular K+ accumulation (salt-in strategy).

  • DOI:10.1038/s41559-024-02505-6 Canonical salt-in strategy accumulates molar cytoplasmic K+ (up to 4M) in hypersaline systems.
• salt-in physiology results in acidic proteome

  Salt-in physiology drives proteome acidification via enrichment in acidic amino acids.

  • DOI:10.1038/s41559-024-02505-6 K+ accumulation accompanied by proteome acidification through Glu/Asp enrichment.
• acidic amino acid enrichment (Asp/Glu) contributes to protein function at very high ionic strength RO:0002326

  Asp/Glu enrichment produces negatively charged surfaces enabling protein function at high ionic strength.

  • DOI:10.1093/femsre/fuy026 Salt-in proteins more hydrophilic and enriched in acidic residues, enabling functionality at very high ionic strength.
• salt-in physiology depends on intracellular K+ RO:0002502

  Salt-in extreme-halophile physiology depends on high intracellular K+ (proteins misfold below ~2.2 M).

  • DOI:10.1093/femsre/fuy026 Cellular vitality of salt-in organisms dependent on high intracellular K+; proteins misfold when K+ falls below ~2.2 M.
• intracellular compatible solutes stabilizes protein stabilization under salt stress

  Intracellular compatible solutes stabilize proteins/enzymes under salt stress.

  • DOI:10.1007/978-3-030-18975-4_4 Osmolytes act as protein stabilizers/chaperones under salt stress.