GC mid2

Edge evidence

• moderate GC-biased gene conversion produces GC mid2 METPO:2000202

  Moderate GC-biased gene conversion yields mid-high genome-wide GC composition.

  • DOI:10.1186/1471-2148-10-374 GC-biased gene conversion Supports GC-biased gene conversion as the driver of mid-high GC bins.
• GC mid2 is a GC content rdfs:subClassOf

  GC mid2 is a quantitative bin of the GC-content phenotype.

  • DOI:10.1038/nrg2358 GC content Supports the 57.0–66.3% bin as a value within the GC-content distribution.
• homologous recombination positively influences moderate GC-biased gene conversion

  Recombination increases fixation of AT->GC mutations via GC-biased gene conversion.

  • DOI:10.1101/011023 gBGC: recombination tends to increase fixation of AT->GC mutations.
• mutation bias toward AT negatively influences GC mid2

  Universal AT-biased mutation opposes maintenance of mid-high genomic GC.

  • DOI:10.1101/011023 Universal excess of G/C->A/T mutations; mutation universally biased towards AT.
• cytosine deamination positively influences mutation bias toward AT

  Cytosine deamination (C->T) drives A+T enrichment.

  • DOI:10.63635/mrj.v1i4.188 Cytosine deamination (C->T) drives A+T enrichment.
• guanine oxidation / 8-oxoG damage positively influences mutation bias toward AT

  Guanine oxidation to 8-oxo-guanine (G->T) contributes to AT-biased composition.

  • DOI:10.63635/mrj.v1i4.188 Guanine oxidation to 8-oxo-guanine (G->T).
• DNA repair efficiency influences GC mid2

  Variation in repair efficiency affects fixation of changes and long-term G+C content.

  • DOI:10.63635/mrj.v1i4.188 Variations in DNA repair efficiency influence fixation and thus long-term G+C content.
• sequencing/assembly GC bias can distort measurement of GC mid2

  Assembly conditions can bias observed GC% in a direction dependent on true GC%.

  • DOI:10.1186/s12864-023-09910-4 In low-GC genomes factors inflate assembly GC; in high-GC genomes they decrease it.