denitrification

Edge evidence

• nitrate oxidized to dinitrogen METPO:2007405

  Nitrate is reduced stepwise via NO2-, NO, N2O to N2.

  • DOI:10.1128/mmbr.61.4.533-616.1997 Zumft reviews the stepwise respiratory reduction of nitrate to N2.
• denitrification participates in anaerobic respiration biolink:participates_in

  Denitrification is a nitrate-respiring form of anaerobic respiration.

  • DOI:10.1038/nrmicro.2018.9 Kuypers et al. place denitrification within the microbial nitrogen-cycling network.
• respiratory nitrate reductase NarGHI enables nitrate reduction to nitrite RO:0002327

  Respiratory nitrate reductase NarGHI catalyzes the first denitrification step.

  • DOI:10.1186/s40793-024-00643-9 narG (NO3- reductase) supports the canonical first step of denitrification.
• cytochrome cd1 nitrite reductase NirS enables nitrite reduction to nitric oxide RO:0002327

  Cytochrome cd1 nitrite reductase NirS reduces nitrite to nitric oxide.

  • DOI:10.1093/ismeco/ycae020 Nitrite is reduced to gaseous NO by nitrite reductases including cytochrome cd1 NirS; strong general mechanistic support.
• nitric oxide reductase Nor enables nitric oxide reduction to nitrous oxide RO:0002327

  Nitric oxide reductase Nor reduces NO to N2O.

  • DOI:10.3389/fmicb.2023.1218207 NO reductase (NOR), encoded by norB, reduces NO to N2O; canonical N2O-producing step.
• nitrous oxide reductase NosZ enables nitrous oxide reduction to dinitrogen RO:0002327

  Nitrous oxide reductase NosZ reduces N2O to N2, the terminal step.

  • DOI:10.3389/fmicb.2023.1218207 N2O reductase (NOS) catalyzes reduction of N2O to N2; canonical terminal step.
• nitrous oxide reductase NosZ part_of denitrification

  NosZ is the final-step component of complete denitrification.

  • DOI:10.3389/fmicb.2024.1407573 Reduction of N2O to N2 requires nitrous oxide reductase encoded by nosZ.
• oxygen (O2) negatively regulates denitrification

  Oxygen acts as a superordinate repressor of denitrification.

  • DOI:10.1038/s41467-024-51688-w O2 acts as a superordinate repressor of denitrification.
• denitrification pathway modularity causes transient accumulation of intermediates biolink:causes

  Modularity of the denitrification pathway drives transient accumulation of intermediates.

  • DOI:10.1038/s41467-024-51688-w The modular nature of the denitrification pathway affects phenotypes and transient accumulation of intermediates.