cr1_diatom_medium

CultureMech:000152 algae COMPLEX LIQUID
Original name: CR1+ Diatom Medium

Overview

Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
Synonyms
  • {'name': '2x_soil_seawater_medium', 'source': 'unknown', 'original_category': 'algae'}
  • {'name': 'ag_diatom_medium', 'source': 'unknown', 'original_category': 'algae'}
  • {'name': 'combo_diatom_medium', 'source': 'unknown', 'original_category': 'algae'}
  • {'name': 'polytomella_medium', 'source': 'unknown', 'original_category': 'algae'}
  • {'name': 'soilwater_peat_medium', 'source': 'unknown', 'original_category': 'algae'}
  • {'name': 'cr1_s_diatom_medium', 'source': 'unknown', 'original_category': 'algae'}
  • {'name': 'soil_extract_sodium_metasilicate_medium', 'source': 'unknown', 'original_category': 'algae'}
  • {'name': 'soilwater_gr_medium', 'source': 'unknown', 'original_category': 'algae'}

Composition

flowchart LR
    CultureMech_000152["cr1_diatom_medium"]
    ing0_1["1"]
    CultureMech_000152 --> ing0_1
    ing1_2["2"]
    CultureMech_000152 --> ing1_2
    ing2_3["3"]
    CultureMech_000152 --> ing2_3

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 1 variable G_PER_L Original amount: Green House Soil
2 2 variable G_PER_L Original amount: CaCO3(Fisher C 64)
3 3 variable G_PER_L Original amount: Supplemented Seawater

Preparation

  1. Important Notes To Consider: The quality of water, including natural seawater, is important.
  2. The term 'dH2O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc.
  3. Natural seawater and natural freshwater should be obtained from a non-polluted source. When dissolving chemicals, wait for the first component to dissolve before adding the second.
  4. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently.
  5. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts. Attention should be given to the pH of the final medium.
  6. In most cases, if pH adjustment is required, this occurs before sterilization.
  7. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers.
  8. This would make the medium very alkaline soon after removal from the autoclave.
  9. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.

Target organisms

No target organisms are recorded.

Provenance

Merged from 10 source records.

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 utex-import Imported from UTEX Culture Collection Source ID: cr1-plus-diatom-medium, URL: https://utex.org/products/cr1-plus-diatom-medium
2026-03-13T06:18:53.305970Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000152
2026-03-17T19:45:04.251136+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CR1+ Diatom Medium" to "cr1_diatom_medium" for consistent matching
2026-05-16T09:02:55.931477+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.125442+00:00 migrate_preparation_steps.py MIGRATED_PREPARATION_STEPS instruction->action+description for 9 step(s)
2026-08-06T03:04:19.301091+00:00 recipe-merger Merged 10 duplicate recipes into canonical record Sources: 2x_soil_seawater_medium, Ag_Diatom_Medium, CR1+_Diatom_Medium, CR1_Diatom_Medium, Combo_Diatom_Medium, Polytomella_Medium, Soilwater_Peat_Medium, cr1_s_diatom_medium, soil_extract_sodium_metasilicate_medium, soilwater_gr_medium
2026-08-06T03:04:19.301149+00:00 merge_recipes.py MERGED_RECIPES merged 10 source recipe(s) on fingerprint 7ee74ca6db4b367eec57934b47ce1e5b6df3bec871194875b45bb7de23168c0b