modified_halobacteria_medium_3

CultureMech:002192 archaea COMPLEX LIQUID
Original name: MODIFIED HALOBACTERIA MEDIUM-3

Overview

External ontology
mediadive.medium:J1008 (source link unavailable) — MODIFIED HALOBACTERIA MEDIUM-3
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'halobacteria_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J168', 'original_category': 'archaea'}
  • {'name': 'halobacteria_medium_ph_8_0', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J1132', 'original_category': 'archaea'}
  • {'name': 'halobacteria_medium_with_15_nacl', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J914', 'original_category': 'archaea'}
  • {'name': 'halobacterium_tmm_mediium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J787', 'original_category': 'archaea'}
  • {'name': 'modified_halobacteria_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J572', 'original_category': 'archaea'}
  • {'name': 'modified_halobacteria_medium_2', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J1007', 'original_category': 'archaea'}
  • {'name': 'lentibacillus_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J377', 'original_category': 'bacterial'}
pH
7.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1008

Composition

flowchart LR
    CultureMech_002192["modified_halobacteria_medium_3"]
    ing0_Casamino_acids["Casamino acids"]
    CultureMech_002192 --> ing0_Casamino_acids
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002192 --> ing1_Yeast_extract
    ing2_Na_glutamate["Na glutamate"]
    CultureMech_002192 --> ing2_Na_glutamate
    CHEBI_64220["CHEBI:64220"]
    ing2_Na_glutamate -.-> CHEBI_64220
    ing3_Trisodium_citrate["Trisodium citrate"]
    CultureMech_002192 --> ing3_Trisodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing3_Trisodium_citrate -.-> CHEBI_53258
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002192 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_KCl["KCl"]
    CultureMech_002192 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_NaCl["NaCl"]
    CultureMech_002192 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002192 --> ing7_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing7_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002192 --> ing8_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing8_MnCl2_x_4_H2O -.-> CHEBI_86368

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Casamino acids 5 G_PER_L
2 Yeast extract 5 G_PER_L
3 Na glutamate
C5H8NO4.Na 169.112 g/mol
1 G_PER_L CHEBI:64220
monosodium glutamate
4 Trisodium citrate
C6H5O7.3Na 258.069 g/mol
3 G_PER_L CHEBI:53258
sodium citrate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
20 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 KCl
Cl.K 74.551 g/mol
2 G_PER_L CHEBI:32588
potassium chloride
7 NaCl
Cl.Na 58.443 g/mol
200 G_PER_L CHEBI:26710
sodium chloride
8 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
36 MG_PER_L CHEBI:86249
iron dichloride tetrahydrate
9 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.36 MG_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate

Preparation

  1. Use Medium No. 168 supplemented with 270.0 g/L NaCl (final) and 15.0 g/L MgSO4 x 7H2O. Adjust pH to 7.5.

Target organisms

No target organisms are recorded.

Provenance

Merged from 8 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:18.301275Z mediadive-import Imported from MediaDive Source: JCM, ID: J1008
2026-02-03T11:26:52.545243 quality-tagger-v1.0 Added data quality flag Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:19:11.118644Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002192
2026-03-17T19:45:23.870146+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MODIFIED HALOBACTERIA MEDIUM-3" to "modified_halobacteria_medium_3" for consistent matching
2026-04-04T18:49:19.145578 copy_referenced_compositions RESOLVED_REFERENCE Resolved reference by copying from halobacteria_medium (CultureMech:002527)
2026-05-16T08:58:09.570492+00:00 migrate_data_quality_flags.py MIGRATED_DATA_QUALITY_FLAGS dict(2 keys) -> list(1 entries)
2026-06-05T07:41:36.684686+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:48.808854+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-08-06T03:04:20.762228+00:00 recipe-merger Merged 8 duplicate recipes into canonical record Sources: JCM_J168_HALOBACTERIA_MEDIUM, halobacteria_medium_ph_8_0, halobacteria_medium_with_15_nacl, halobacterium_tmm_mediium, modified_halobacteria_medium, modified_halobacteria_medium_2, modified_halobacteria_medium_3, JCM_J377_LENTIBACILLUS_MEDIUM
2026-08-06T03:04:20.762292+00:00 merge_recipes.py MERGED_RECIPES merged 8 source recipe(s) on fingerprint c5e4cbeb7a3dfb3de662bb55f20fd19f6fa98950f51d5a7b44382a4103953693