mesophilic

Edge evidence

• moderate ambient temperature selects for mesophilic METPO:2007401

  Moderate ambient temperatures select for mesophilic physiology.

  • DOI:10.1016/j.bpj.2013.06.029 Escherichia coli, a mesophilic bacterium Supports a representative mesophile thriving at moderate temperatures.
• homoviscous lipid composition regulates membrane fluidity RO:0002211

  Homoviscous lipid composition maintains target membrane fluidity at mesophilic temperatures.

  • DOI:10.1146/annurev-micro-091313-103612 more unsaturated fatty acids Supports homoviscous adaptation as the mechanism setting membrane fluidity.
• mesophile enzyme repertoire enables balanced mesophilic growth RO:0002327

  Mesophile enzyme repertoire enables balanced growth at intermediate temperatures.

  • DOI:10.1016/s0300-9629(97)00003-0 energy transducing enzymes Supports temperature-tuned enzyme repertoires as the basis of adapted growth.
• balanced mesophilic growth manifests as mesophilic METPO:2007400

  Balanced growth at moderate temperatures manifests the mesophilic trait.

  • DOI:10.1016/j.bpj.2013.06.029 Escherichia coli, a mesophilic bacterium Supports the trait endpoint in a representative organism.
• temperature decrease increases unsaturated fatty acids RO:0002213

  Temperature downshift increases membrane unsaturated fatty acid content (homeoviscous adaptation).

  • DOI:10.1007/s42770-023-01057-4 Low-temperature adaptation includes increased unsaturation of membrane acyl chains; broad bacterial homeoviscous adaptation.
• cold shock induces CspA cold-shock protein

  Cold shock induces the cold-shock protein CspA.

  • DOI:10.1007/s12275-023-00031-x Cold shock responses include induction of Csp proteins, notably CspA (~15% of protein synthesis after cold shock).
• CspA cold-shock protein promotes translation RO:0002213

  CspA acts as an RNA chaperone preventing RNA secondary structure, promoting translation during cold shock.

  • DOI:10.1007/s12275-023-00031-x CspA binds/unwinds RNA to promote single-strandedness and translation during cold shock.
• heat shock induces sigma-32 (RpoH)

  Heat shock induces synthesis of sigma-32 (RpoH).

  • DOI:10.1007/s12275-023-00031-x Heat-induced synthesis of sigma-32 (rpoH); canonical bacterial heat-shock regulation relevant to mesophiles near the upper temperature range.
• sigma-32 (RpoH) activates expression of heat shock genes

  Sigma-32 (RpoH) activates expression of heat-shock genes.

  • DOI:10.1007/s12275-023-00031-x Heat shock regulation centers on sigma factor RpoH (sigma-32) directing heat-shock gene expression.
• DnaK chaperone negatively regulates sigma-32 (RpoH)

  DnaK chaperone sequesters sigma-32, negatively regulating its activity.

  • DOI:10.1007/s12275-023-00031-x RpoH is controlled by DnaK chaperone sequestration; canonical negative regulation in Gram-negative bacteria.