soilwater_gr_nh4_medium (CultureMech:000228)

Overview

Applications

Algae cultivation
Phytoplankton culture
Microalgae research

Synonyms

{'name': '1_2_enriched_seawater', 'source': 'unknown', 'original_category': 'algae'}
{'name': '1_2_erdschreiber_medium', 'source': 'unknown', 'original_category': 'algae'}
{'name': '1_4_erdschreibers_medium', 'source': 'unknown', 'original_category': 'algae'}
{'name': '20_allen_80_erdschreiber_1_4_medium', 'source': 'unknown', 'original_category': 'algae'}
{'name': '2_3_enriched_seawater', 'source': 'unknown', 'original_category': 'algae'}
{'name': '5_3_soil_seawater_agar_medium', 'source': 'unknown', 'original_category': 'algae'}
{'name': 'bold_1nv_erdshreiber_1_1_medium', 'source': 'unknown', 'original_category': 'algae'}
{'name': 'bold_1nv_erdshreiber_4_1_medium', 'source': 'unknown', 'original_category': 'algae'}
{'name': 'botryococcus_medium', 'source': 'unknown', 'original_category': 'algae'}
{'name': 'es_10_enriched_seawater_medium', 'source': 'unknown', 'original_category': 'algae'}
{'name': 'es_2_enriched_seawater_medium', 'source': 'unknown', 'original_category': 'algae'}
{'name': 'es_4_enriched_seawater_medium', 'source': 'unknown', 'original_category': 'algae'}
{'name': 'enriched_seawater_medium', 'source': 'unknown', 'original_category': 'algae'}
{'name': 'proteose_medium', 'source': 'unknown', 'original_category': 'algae'}
{'name': 'ss_diatom_medium', 'source': 'unknown', 'original_category': 'algae'}
{'name': 'soil_extract_medium', 'source': 'unknown', 'original_category': 'algae'}
{'name': 'soilwater_gr_medium', 'source': 'unknown', 'original_category': 'algae'}
{'name': 'soilwater_vt_medium', 'source': 'unknown', 'original_category': 'algae'}
{'name': 'spirulina_medium', 'source': 'unknown', 'original_category': 'algae'}
{'name': 'volvocacean_medium', 'source': 'unknown', 'original_category': 'algae'}
{'name': 'bg_11_0_36_nacl_medium', 'source': 'unknown', 'original_category': 'algae'}
{'name': 'bg_11_1_nacl_medium', 'source': 'unknown', 'original_category': 'algae'}
{'name': 'bristol_nacl_medium', 'source': 'unknown', 'original_category': 'algae'}
{'name': 'chus_medium', 'source': 'unknown', 'original_category': 'algae'}
{'name': 'f_2_nh4_medium', 'source': 'unknown', 'original_category': 'algae'}
{'name': 'soil_seawater_medium', 'source': 'unknown', 'original_category': 'algae'}
{'name': 'waris_soil_extract_medium', 'source': 'unknown', 'original_category': 'algae'}

Composition

flowchart LR
    CultureMech_000228["soilwater_gr_nh4_medium"]
    ing0_1["1"]
    CultureMech_000228 --> ing0_1
    ing1_2["2"]
    CultureMech_000228 --> ing1_2

Ingredients (2)

#	Ingredient	Concentration	Ontology	Notes
1	1	variable G_PER_L		Original amount: Enriched Seawater Medium
2	2	variable G_PER_L		Original amount: sterile dH2O

Preparation

Important Notes To Consider: The quality of water, including natural seawater, is important.
The term 'dH2O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc.
Natural seawater and natural freshwater should be obtained from a non-polluted source. When dissolving chemicals, wait for the first component to dissolve before adding the second.
Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently.
Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts. Attention should be given to the pH of the final medium.
In most cases, if pH adjustment is required, this occurs before sterilization.
Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers.
This would make the medium very alkaline soon after removal from the autoclave.
In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.

Target organisms

No target organisms are recorded.

Provenance

Merged from 29 source records.

1_2_Enriched_Seawater (CultureMech:000003)
1_2_Erdschreiber_Medium (CultureMech:000004)
1_4_erdschreibers_medium (CultureMech:000008)
20_allen_80_erdschreiber_1_4_medium (CultureMech:000013)
2_3_Enriched_Seawater (CultureMech:000018)
5_3_soil_seawater_agar_medium (CultureMech:000019)
Bold_1NV_Erdshreiber_1_1_Medium (CultureMech:000030)
Bold_1NV_Erdshreiber_4_1_Medium (CultureMech:000031)
Botryococcus_Medium (CultureMech:000035)
ES_10_Enriched_Seawater_Medium (CultureMech:000163)
ES_2_Enriched_Seawater_Medium (CultureMech:000164)
ES_4_Enriched_Seawater_Medium (CultureMech:000165)
Enriched_Seawater_Medium (CultureMech:000166)
Proteose_Medium (CultureMech:000188)
SS_Diatom_Medium (CultureMech:000219)
Soil_Extract_Medium (CultureMech:000223)
Soilwater_GR-_Medium (CultureMech:000227)
Soilwater_GR-_NH4_Medium (CultureMech:000228)
Soilwater_VT_Medium (CultureMech:000231)
Spirulina_Medium (CultureMech:000232)
Volvocacean_Medium (CultureMech:000236)
bg_11_0_36_nacl_medium (CultureMech:000026)
bg_11_1_nacl_medium (CultureMech:000027)
bristol_nacl_medium (CultureMech:000036)
chus_medium (CultureMech:000155)
f_2_nh4_medium (CultureMech:000169)
soil_seawater_medium (CultureMech:000221)
soilwater_gr_nh4_medium (CultureMech:000226)
waris_soil_extract_medium (CultureMech:000241)

Timestamp	Curator	Action	Notes
2026-01-28T00:00:00+00:00	utex-import	Imported from UTEX Culture Collection	Source ID: soilwater-gr-minus-nh4-medium, URL: https://utex.org/products/soilwater-gr-minus-nh4-medium
2026-03-13T06:18:53.633957Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000228
2026-03-17T19:45:04.548134+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Soilwater: GR-/NH4 Medium" to "soilwater_gr_nh4_medium" for consistent matching
2026-05-16T09:02:56.124839+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.338686+00:00	migrate_preparation_steps.py	MIGRATED_PREPARATION_STEPS	instruction->action+description for 9 step(s)
2026-08-06T03:04:19.186667+00:00	recipe-merger	Merged 29 duplicate recipes into canonical record	Sources: 1_2_Enriched_Seawater, 1_2_Erdschreiber_Medium, 1_4_erdschreibers_medium, 20_allen_80_erdschreiber_1_4_medium, 2_3_Enriched_Seawater, 5_3_soil_seawater_agar_medium, Bold_1NV_Erdshreiber_1_1_Medium, Bold_1NV_Erdshreiber_4_1_Medium, Botryococcus_Medium, ES_10_Enriched_Seawater_Medium, ES_2_Enriched_Seawater_Medium, ES_4_Enriched_Seawater_Medium, Enriched_Seawater_Medium, Proteose_Medium, SS_Diatom_Medium, Soil_Extract_Medium, Soilwater_GR-_Medium, Soilwater_GR-_NH4_Medium, Soilwater_VT_Medium, Spirulina_Medium, Volvocacean_Medium, bg_11_0_36_nacl_medium, bg_11_1_nacl_medium, bristol_nacl_medium, chus_medium, f_2_nh4_medium, soil_seawater_medium, soilwater_gr_nh4_medium, waris_soil_extract_medium
2026-08-06T03:04:19.186795+00:00	merge_recipes.py	MERGED_RECIPES	merged 29 source recipe(s) on fingerprint 22074227d8462b39403011e0bc4c5e7a3f1ee1bae54ae2deb0943dece537f93f