Methanogenesis

Edge evidence

• Methanogenesis occurs in anoxic habitat biolink:occurs_in

  Hydrogenotrophic methanogenesis is associated with anoxic habitats.

  • DOI:10.1146/annurev-micro-011720-122807 terminal step of microbial biomass degradation in anoxic habitats Review places hydrogenotrophic methanogenesis in anoxic microbial biomass degradation.
• carbon dioxide reduced with electron donor molecular hydrogen

  Carbon dioxide is reduced using electrons derived from hydrogen.

  • DOI:10.1146/annurev-micro-011720-122807 from CO2 and H2 to methane Supports the CO2 plus H2 substrate relationship.
• methyl-coenzyme M reductase has prosthetic group coenzyme F430

  Methyl-coenzyme M reductase contains nickel coenzyme F430.

  • DOI:10.1021/acs.biochem.9b00164 harbors the nickel(I) hydrocorphin coenzyme F-430 Supports F430 as the prosthetic group of the enzyme.
• methyl-coenzyme M reductase catalyzes formation of methane biolink:catalyzes

  Methyl-coenzyme M reductase catalyzes the terminal methane-forming reaction.

  • DOI:10.1021/acs.biochem.9b00164 catalyzes the reversible reduction of methyl-coenzyme M Source describes methane formation by methyl-coenzyme M reductase.
• Methanogenesis produces methane METPO:2000202

  The defining output of methanogenesis is methane.

  • DOI:10.1146/annurev-micro-011720-122807 from CO2 and H2 to methane Supports methane as the reduced product of hydrogenotrophic methanogenesis.
• mcrABG gene cluster enables methyl-coenzyme M reductase RO:0002327

  The mcrABG gene cluster encodes the methyl-coenzyme M reductase complex.

  • DOI:10.1128/mmbr.00024-22 A key genetic requirement across these pathways is the presence of mcrABG.
• acetate serves as substrate for acetoclastic methanogenesis

  Acetate is the substrate split to methane and CO2 in acetoclastic methanogenesis.

  • DOI:10.1007/s00253-023-12700-3 acetoclastic (splitting acetate to CH4 and CO2).
• methylated compounds serves as substrate for methyl-based methanogenesis

  Methylated compounds are the substrates for methyl-based methanogenesis.

  • DOI:10.1007/s00253-023-12700-3 methylotrophic (using methylated compounds).
• coenzyme B is required for methane

  Coenzyme B reacts with methyl-CoM in the terminal step to yield methane.

  • DOI:10.1128/mmbr.00024-22 methyl-CoM reacts with coenzyme B (CoB) to yield methane.
• coenzyme M carries methyl group in Methanogenesis

  Methyltransferases transfer methyl groups to coenzyme M to form methyl-CoM.

  • DOI:10.1128/mmbr.00024-22 methyltransferases transfer methyl groups ultimately to CoM to form methyl-CoM.
• coenzyme F420 (F420H2) donates electrons to carbon dioxide METPO:2007403

  Reduced coenzyme F420 donates electrons for reduction of C1 intermediates derived from CO2.

  • DOI:10.3389/fmicb.2023.1296008 Reduced coenzyme F420 (F420H2) donates electrons for reductions of methenyl/methylene intermediates.