non motile

Edge evidence

• absent or non-expressed flagellum prevents no propulsion RO:0002212

  Without a flagellum cells cannot generate flagellar propulsion.

  • DOI:10.1146/annurev.micro.57.030502.090832 flagellum Supports the flagellum as the major bacterial motility organelle.
• absent motility pili prevents no propulsion RO:0002212

  Without motility pili cells cannot generate twitching or related pilus-driven movement.

  • DOI:10.1038/nrmicro2218 type IV pili Type-IV-pili review supports type IV pili as the cellular basis for twitching motility (and their absence as the basis for non-twitching/non-motile phenotype).
• no propulsion manifests as non motile METPO:2007400

  Lack of propulsion manifests the non-motile trait.

  • DOI:10.3389/fmicb.2025.1514643 They are Gram-negative, non-motile rods Supports the trait endpoint in a representative organism.
• disrupted flagellum biogenesis prevents no propulsion RO:0002212

  Disrupted flagellum biogenesis yields a non-functional flagellum and loss of propulsion.

  • DOI:10.1186/s12866-024-03387-1 flagellum biogenesis was affected in mutants and lack of functional flagellum confirmed in motility assays.
• WspR diguanylate cyclase increases amount of high intracellular cyclic di-GMP

  WspR diguanylate cyclase produces c-di-GMP when phosphorylated, raising intracellular levels.

  • DOI:10.1128/aem.01548-23 WspR produces c-di-GMP when phosphorylated; high c-di-GMP represses motility.
• high intracellular cyclic di-GMP causes repression of flagellar gene expression biolink:causes

  High c-di-GMP causes FleQ to cease activating flagellar genes, repressing flagellar gene expression.

  • DOI:10.1128/jb.00365-23 At high c-di-GMP, FleQ ceases to activate flagellar genes.
• flhDC flagellar master operon positively regulates repression of flagellar gene expression

  Repression of the flhDC master operon decreases flagellar gene expression; included as the master-regulator control point.

  • DOI:10.3390/foods13223709 OmpR binds the flhDC operon promoter and represses transcription, decreasing flagellar gene expression and motility.
• repression of flagellar gene expression causes reduced or absent motility biolink:causes

  Repression of flagellar genes leads to reduced or absent motility.

  • DOI:10.1128/jb.00365-23 Loss of flagellar gene activation lowers motility, which can manifest as a non-motile state.
• reduced or absent motility manifests as non motile METPO:2007400

  Sufficiently reduced/absent motility manifests the non-motile trait.

  • DOI:10.1128/aem.01548-23 Elevated c-di-GMP regulatory output yields a non-motile mutant phenotype.