non-spore forming

Edge evidence

• absent Spo0A regulatory cascade prevents no asymmetric septation RO:0002212

  Without the Spo0A/sigma cascade, asymmetric septation cannot be initiated.

  • DOI:10.1146/annurev.genet.30.1.297 activation of these sigma factors to landmark events in morphogenesis Supports the Spo0A/sigma cascade as the gatekeeper of sporulation morphogenesis.
• no asymmetric septation prevents no endospore formation RO:0002212

  Without asymmetric septation, the forespore compartment and subsequent endospore cannot be produced.

  • DOI:10.1146/annurev.genet.30.1.297 activation of these sigma factors to landmark events in morphogenesis Supports asymmetric septation as a required step for endospore formation.
• no endospore formation manifests as non-spore forming METPO:2007400

  Failure to produce endospores manifests the non-spore-forming trait.

  • DOI:10.1155/2013/898106 S. aureus does not form spores Supports the trait endpoint in a representative organism.
• absence of spo0A gene predicts non-spore forming

  Absence of spo0A is an excellent predictor of inability to sporulate.

  • DOI:10.1128/jb.00079-22 Comparative genomics of 180 genomes: spo0A present in all 76 sporeformers; its absence predicts non-sporulation.
• loss of sporulation genes causes non-spore forming biolink:causes

  Loss of a considerable fraction of sporulation genes yields an asporogenic phenotype.

  • DOI:10.1111/1462-2920.16145 Asporogenic phenotypes can result from inactivation or loss of a considerable fraction of sporulation genes (generic edge).
• low or absent Spo0A activity causes loss of sporulation capacity biolink:causes

  Low or absent Spo0A activity abolishes sporulation capacity.

  • DOI:10.3389/fmicb.2021.630573 Spo0A is the master regulator of endospore formation; elevated Spo0A~P is required to trigger sporulation.
• loss of sporulation capacity manifests as non-spore forming METPO:2007400

  Loss of sporulation capacity manifests the non-spore-forming phenotype.

  • DOI:10.3389/fmicb.2021.630573 Failure to produce/activate Spo0A prevents sporulation, yielding the non-spore-forming trait.
• Rap phosphatases dephosphorylates dephosphorylation of Spo0F

  Rap phosphatases directly dephosphorylate Spo0F.

  • DOI:10.1038/s41522-024-00594-6 Rap phosphatases directly dephosphorylate Spo0F (generic to Bacillus phosphorelay logic).
• dephosphorylation of Spo0F decreases activation of low or absent Spo0A activity

  Dephosphorylation of Spo0F reduces phosphate flow to Spo0A, decreasing its activation.

  • DOI:10.1038/s41522-024-00594-6 Reduced phosphate flow to Spo0A in the sporulation phosphorelay (corroborated by DOI:10.3390/microorganisms11081928).
• Spo0A phosphorelay disruption prevents no entry into sporulation RO:0002212

  Disruption of the KinA/KinB -> Spo0F -> Spo0B -> Spo0A phosphorelay arrests entry into sporulation.

  • DOI:10.3390/microorganisms11081928 The phosphorelay signals the start of sporulation; mutants arrest at stages 0/I when initiation fails.
• no entry into sporulation manifests as non-spore forming METPO:2007400

  Failure to enter sporulation manifests the non-spore-forming phenotype.

  • DOI:10.3390/microorganisms11081928 Arrest at sporulation initiation produces the non-spore-forming endpoint.