vibrio shaped

Edge evidence

• CrvA self-assembles into periplasmic filament

  CrvA forms periplasmic filaments at the inner face of cell curvature.

  • DOI:10.1016/j.cell.2016.12.019 CrvA self-assembles into filaments at the inner face Supports CrvA as a periplasmic polymer at the inner curvature.
• periplasmic filament patterns asymmetric peptidoglycan insertion

  CrvA filaments pattern differential peptidoglycan insertion.

  • DOI:10.1016/j.cell.2016.12.019 asymmetrically patterns peptidoglycan insertion Supports CrvA-dependent spatial patterning of wall insertion.
• asymmetric peptidoglycan insertion causes cell-body curvature biolink:causes

  Unequal peptidoglycan insertion creates more material on the outer face than the inner face, curving the cell.

  • DOI:10.1016/j.cell.2016.12.019 causing more material insertions into the outer face Supports asymmetric wall insertion as the proximal cause of curvature.
• cell-body curvature produces vibrio shaped METPO:2000202

  Curved rod morphology produces the vibrioid/comma-shaped trait.

  • DOI:10.1016/j.cell.2016.12.019 characteristic curved rod morphology Supports the observed Vibrio morphology as a curved-rod shape.
• CrvB increases cell-body curvature RO:0002213

  CrvB expression increases CrvA assembly and cell curvature in a dose-dependent manner.

  • DOI:10.1101/2020.02.20.954503 crvB expression increased CrvA-GFP assembly as well as cell curvature in a dose-dependent manner.
• CrvB colocalizes with periplasmic filament

  CrvB colocalizes with CrvA in the same periplasmic inner-curve filament structure.

  • DOI:10.1101/2020.02.20.954503 Both proteins localized to the same structure; CrvB-GFP filaments colocalized with periplasmic DsbA-mCherry.
• VadR sRNA represses crvA mRNA

  VadR sRNA is a post-transcriptional inhibitor of crvA mRNA.

  • DOI:10.1038/s41467-020-19890-8 The VadR small RNA acts as a post-transcriptional inhibitor of the crvA mRNA.
• crvA mRNA translated into CrvA

  crvA mRNA is translated into the CrvA curvature determinant protein.

  • DOI:10.1038/s41467-020-19890-8 crvA mRNA encodes the periplasmic CrvA protein that determines cell curvature.
• VadR sRNA decreases cell-body curvature RO:0002212

  Mutation of vadR increases cell curvature whereas overexpression has the inverse effect, indicating VadR limits curvature.

  • DOI:10.1038/s41467-020-19890-8 Mutation of vadR increases cell curvature, whereas overexpression has the inverse effect.
• VxrAB two-component system activates transcription of VadR sRNA

  The VxrAB two-component system activates vadR transcription.

  • DOI:10.1038/s41467-020-19890-8 vadR transcription is activated by the VxrAB two-component system.
• cyclic di-GMP decreases cell-body curvature RO:0002212

  High c-di-GMP concentrations decrease cell curvature to generate straight rods.

  • DOI:10.1073/pnas.2010199117 High c-di-GMP concentrations decrease cell curvature to generate straight rods.