oxidase activity

Edge evidence

• cytochrome c oxidase enables cytochrome-c oxidase activity RO:0002327

  Cytochrome c oxidase carries out terminal oxidase activity.

  • DOI:10.3390/microorganisms10050926 Hederstedt reviews bacterial cytochrome c oxidase.
• cytochrome-c oxidase activity consumes molecular oxygen biolink:consumes

  The terminal oxidase reduces molecular oxygen to water.

  • DOI:10.1089/ars.2020.8039 Borisov et al. review terminal oxidases that reduce O2.
• cytochrome c oxidase enables oxidase activity RO:0002327

  Possession of a terminal oxidase confers the oxidase-activity phenotype.

  • DOI:10.3390/microorganisms10050926 Supports the oxidase-test basis.
• reduced cytochrome c donates electrons to CuA center METPO:2007403

  Reduced cytochrome c donates electrons to the CuA center of subunit II.

  • DOI:10.3390/microorganisms10050926 Hederstedt: "reduced cytochrome c donates electrons to the CuA center in subunit II."
• CuA center transfers electrons to heme a METPO:2007403

  The CuA center relays electrons to low-spin heme a.

  • DOI:10.3390/microorganisms10050926 Hederstedt: electrons pass from CuA "then to low-spin heme a."
• heme a3-CuB binuclear center reduces molecular oxygen METPO:2000017

  The heme a3-CuB binuclear center reduces molecular oxygen to water.

  • DOI:10.3390/microorganisms10050926 Hederstedt: "the dioxygen reduction site (heme a3 + CuB)."
• family A cytochrome c oxidase has cofactor heme a

  Family A cytochrome c oxidase contains two heme A prosthetic groups (heme a and heme a3).

  • DOI:10.3390/microorganisms10050926 Hederstedt: "contains two heme A prosthetic groups (heme a and heme a3)."
• family A cytochrome c oxidase has cofactor CuA/CuB copper centers

  Family A cytochrome c oxidase carries three copper atoms in CuA and CuB centers.

  • DOI:10.3390/microorganisms10050926 Hederstedt: "three copper atoms (CuA is di-copper in subunit II; CuB in subunit I)."
• carbon monoxide inhibits cytochrome-c oxidase activity RO:0002212

  Carbon monoxide is a potent inhibitor of heme-copper cytochrome c oxidase activity.

  • DOI:10.3390/ijms26062809 Borisov & Forte 2025: "CO is a potent inhibitor of heme-copper cytochrome c oxidase (Ki ~0.3 uM)."