methanotrophic

Edge evidence

• methanotrophic uses substrate methane

  Methane is the defining carbon and energy source for methanotrophy.

  • DOI:10.1039/D3CY00737E methane as carbon and energy sources Review places methane among C1 compounds used by methanotrophs.
• methane monooxygenase oxidizes methane METPO:2000016

  Methane monooxygenase catalyzes methane oxidation.

  • DOI:10.1039/D3CY00737E methane oxidation ... catalyzed by two types of methane monooxygenases Supports MMO as the enzyme for the first oxidation step.
• methane monooxygenase uses oxidant molecular oxygen

  Aerobic methane monooxygenases activate oxygen during methane oxidation.

  • DOI:10.1039/D3CY00737E activation of molecular oxygen Supports oxygen involvement in MMO catalysis.
• methane oxidized to methanol METPO:2007405

  The first aerobic methane oxidation product is methanol.

  • DOI:10.1039/D3CY00737E convert methane to methanol Supports methane-to-methanol conversion by MMO.
• methane monooxygenase located in intracellular membrane biolink:located_in

  Particulate methane monooxygenase is membrane associated in methanotrophs.

  • DOI:10.1039/D3CY00737E pMMO is located in the characteristic intracellular membrane Supports subcellular localization for pMMO.
• methanol dehydrogenase oxidizes methanol METPO:2000016

  Methanol dehydrogenase converts methanol onward to formaldehyde.

  • DOI:10.1039/D3CY00737E Methanol is oxidized to formaldehyde Supports methanol dehydrogenase as the next step.
• formaldehyde participates in formaldehyde assimilation biolink:participates_in

  Formaldehyde can be assimilated through RuMP or serine pathways.

  • DOI:10.1039/D3CY00737E RuMP pathway and the serine pathway Supports formaldehyde assimilation routes in methanotrophs.
• methanotrophic produces carbon dioxide METPO:2000202

  Methane oxidation can continue to carbon dioxide for energy metabolism.

  • DOI:10.1039/D3CY00737E methane cycle between ... methane and CO2 Supports methane oxidation in the methane-carbon dioxide cycle; graph focuses on aerobic methanotrophy.
• copper positively regulates particulate methane monooxygenase (pMMO)

  High copper favors pMMO expression (the copper switch).

  • DOI:10.1039/D3CY00737E high copper favors pMMO and represses sMMO
• copper negatively regulates soluble methane monooxygenase (sMMO)

  High copper represses sMMO; low copper favors sMMO (the copper switch).

  • DOI:10.1039/D3CY00737E high copper favors pMMO and represses sMMO; low copper/biomass favors sMMO
• pmoCAB operon encodes PmoA/PmoB/PmoC subunits biolink:encodes

  pmoCAB operon encodes the PmoA, PmoB and PmoC subunits of pMMO.

  • DOI:10.1039/D3CY00737E pMMO ... composed of subunits PmoA, PmoB, and PmoC, encoded by the pmoCAB operon
• mmoXYZ genes encodes MmoX/MmoY/MmoZ (MMOH alpha/beta/gamma) biolink:encodes

  mmoXYZ genes encode the MMOH alpha/beta/gamma hydroxylase subunits of sMMO.

  • DOI:10.1039/D3CY00737E MMOH is a dimer of heterotrimers encoded by the mmoXYZ genes
• methanobactin binds Cu(I)

  Methanobactin binds Cu(I) with high affinity.

  • DOI:10.1021/acs.chemrev.3c00727 Mbns are ribosomally synthesized ... that bind Cu(I) with particularly high affinity
• methanobactin enables copper acquisition RO:0002327

  Methanobactin-mediated copper chelation enables copper uptake.

  • DOI:10.1039/D3CY00737E some methanotrophs produce methanobactin (high affinity for Cu(I)) for copper collection
• copper acquisition promotes particulate methane monooxygenase (pMMO) RO:0002213

  Copper acquisition supplies the cofactor required for pMMO activity.

  • DOI:10.1021/acs.chemrev.3c00727 As a required cofactor for pMMO activity ... copper is central to methanotroph physiology