lithoautotrophic

Edge evidence

• lithoautotrophic uses electron donor inorganic electron donor METPO:2000009

  Lithoautotrophy uses inorganic compounds as electron donors.

  • DOI:10.1016/B978-0-12-378630-2.00219-X inorganic atoms or molecules as a growth-supporting reductant Supports inorganic electron donors for lithotrophic growth.
• inorganic electron donor enables respiratory energy conservation RO:0002327

  Oxidation of inorganic donors supplies redox energy.

  • DOI:10.1016/j.bbabio.2008.09.008 free energy of a redox reaction Supports energy conservation from respiratory redox reactions.
• respiratory energy conservation generates reducing power biolink:produces

  Lithoautotrophic energy metabolism supplies reducing equivalents for biosynthesis.

  • DOI:10.1016/j.biortech.2021.125768 NADH, or ... NADPH Review discusses reduced redox cofactors generated from chemolithoautotrophic electron donors.
• carbon dioxide fixed by autotrophic CO2 fixation METPO:2007404

  CO2 is assimilated by autotrophic carbon fixation pathways.

  • DOI:10.1128/AEM.02473-10 autotrophic CO2 fixation Supports CO2 fixation as the autotrophic carbon-assimilation process.
• reducing power enables autotrophic CO2 fixation RO:0002327

  Reducing equivalents support conversion of CO2 into cell carbon.

  • DOI:10.1016/j.biortech.2021.125768 reduction of CO2 in the carbon fixation mechanisms Supports reductant use for CO2 reduction.
• autotrophic CO2 fixation produces cellular carbon METPO:2000202

  Autotrophic CO2 fixation produces cellular organic carbon.

  • DOI:10.1128/AEM.02473-10 CO2 ... into cellular carbon Supports assimilation of CO2 into cellular carbon.
• membrane-bound (de)hydrogenase drives electron transport chain

  Membrane-bound donor-oxidizing enzymes reduce the quinone pool and drive the electron transport chain.

  • DOI:10.1128/aem.00748-24 Membrane-bound (de-)hydrogenases couple donor oxidation to quinone reduction which drives the ETC.
• electron transport chain builds proton motive force biolink:produces

  The electron transport chain builds up a proton motive force.

  • DOI:10.1128/aem.00748-24 drives the electron transport chain (ETC) to build up a proton motive force.
• proton motive force drives ATP synthesis

  The proton motive force drives ATP synthesis.

  • DOI:10.1007/s10295-020-02309-0 This process generates a proton motive force to drive ATP synthesis.
• reverse electron flow generates NAD(H) reducing equivalents biolink:produces

  Endergonic reverse electron flow generates NAD(H) for carbon fixation.

  • DOI:10.1007/s10295-020-02309-0 an uphill (endergonic) pathway to generate NAD(H) through reverse electron flow.
• cytochrome bc1 and NADH dehydrogenase mediates reverse electron flow

  Cytochrome bc1 and NADH dehydrogenase mediate reverse electron transfer reducing NAD+ to NAD(H).

  • DOI:10.1007/s10295-020-02309-0 mediated by cytochrome bc1 and NADH dehydrogenase which transfer electrons from the ubiquinone pool to reduce NAD+ to NAD(H).
• carbonic anhydrase and DIC transporters facilitates autotrophic CO2 fixation

  DIC transporters and carbonic anhydrase facilitate dissolved inorganic carbon fixation.

  • DOI:10.1128/aem.01557-23 Autotrophs make use of a toolkit of DIC transporters and carbonic anhydrase enzymes to facilitate DIC fixation; broad across autotrophs.