zinc tolerant

Edge evidence

• zinc(2+) ion challenges zinc tolerant METPO:2007406

  Cytoplasmic zinc excess is the challenge the trait counters.

  • DOI:10.3389/fmicb.2020.00047 Cupriavidus metallidurans BS1 tolerates Zn(2+) to a MIC of 20 mM.
• czc cation-efflux system enables zinc ion transmembrane transport RO:0002327

  CzcP exports zinc together with cadmium and cobalt cations.

  • DOI:10.1111/j.1365-2958.2009.06792.x CzcP exports transition metals Zn(2+), Cd(2+), and Co(2+).
• zinc ion transmembrane transport mitigates zinc(2+) ion METPO:2007407

  Czc-mediated export depletes the cytoplasmic zinc pool.

  • DOI:10.1111/j.1365-2958.2009.06792.x The czc efflux system of C. metallidurans confers zinc resistance.
• ZntA P-type ATPase exports zinc(2+) ion METPO:2000209

  ZntA PIB2-type ATPase effluxes cytoplasmic Zn(2+).

  • DOI:10.1128/jb.00080-24 Efflux of zinc ions is mediated by the PIB2-type ATPase, ZntA.
• ZntA P-type ATPase promotes zinc tolerant RO:0002213

  ZntA-mediated efflux is causal for zinc resistance.

  • DOI:10.1128/jb.00080-24 Deletion of PIB2 ATPases sharply lowers IC50 (to 7.7 uM), showing their causal role in resistance.
• zinc(2+) ion upregulates zntA transcription

  Elevated zinc induces transcription of the zntA efflux gene.

  • DOI:10.1128/jb.00299-24 At external zinc concentrations above 200 nM expression of the zntA gene is upregulated.
• ZntR MerR-family regulator required for zntA transcription

  ZntR is essential for zinc-responsive zntA expression.

  • DOI:10.1128/jb.00299-24 ZntR was essential for zntA expression.
• zntA transcription results in czc cation-efflux system

  zntA transcription yields the ZntA efflux machinery.

  • DOI:10.1128/jb.00299-24 Increased zntA expression provides ZntA exporter under zinc excess.
• CzcCBA RND efflux complex exports zinc(2+) ion METPO:2000209

  CzcCBA RND pump exports Zn(2+) from the periplasm.

  • DOI:10.1128/jb.00080-24 The plasmid-encoded RND transenvelope pump CzcCBA exports Co(II), Zn(II), and Cd(II).
• polyphosphate contributes to zinc ion buffering RO:0002326

  Polyphosphate buffers cytoplasmic zinc, shaping flow equilibrium.

  • DOI:10.1128/jb.00080-24 Metal-binding cytoplasmic components polyphosphate and glutathione influenced the flow equilibrium.
• glutathione contributes to zinc ion buffering RO:0002326

  Glutathione buffers cytoplasmic zinc, shaping flow equilibrium.

  • DOI:10.1128/jb.00080-24 Polyphosphate and glutathione influenced the flow equilibrium.
• zinc ion buffering mitigates zinc(2+) ion METPO:2007407

  Buffering lowers free cytoplasmic zinc.

  • DOI:10.1128/jb.00080-24 Cytoplasmic buffering components influence the zinc flow equilibrium.